The University of Basrah discusses a doctoral thesis on Biochemical and Theoretical Study of Some New Azo-Imine Derivatives

The College of Science in the Department of Chemistry discussed a doctoral thesis entitled: “Biochemical and Theoretical Studies of Some New Azo-Imine Derivatives”. Hold at the College of Science, Chemistry Department. A graduate student (Mohammad Hamad Ati). The thesis aims to synthesis of some new azo-imine derivatives and characterize by FT-IR, ¹HNMR, ¹³CNMR spectroscopy, Mass spectrometry, and X-ray crystallography. After that study of LD50, Antibacterial Activity, Antioxidant Activity, MTT cell viability, Antidiabetic Activity and statistical analysis for the results. Finally, the effectiveness of the compounds was studied theoretically according to DFT, MOE, and MD theories to know their effectiveness and stability, as well as to show the binding of the synthesized compounds with some target proteins for Staphylococcus aureus and MCF-7 cell line.

The study found that the safe concentration of the use of these compounds between 700-850 mg/kg. It also showed efficacy against two strains of gram positive bacteria,  Staphylococcus aureus and Bacillus, while no activity was shown against two strains of gram negative bacteria, Escherichia coli and Pseudomonas aeruginosa. It also gave high efficacy as an antioxidant. While the study showed that MCF-7 cells showed that some compounds showed good efficacy against cancer cells. The study also showed that some compounds reduced the blood sugar level of diabetic rabbits while not affecting the blood glucose level of normal rabbits. The theoretical study also showed the stability of the prepared compounds and their ability to bind to the received proteins (PDB ID: 4URO) of the target bacterial line Staphylococcus aureus as well as with the protein (PDB ID: 3ERT) target protein for MCF-7 cells.

The study recommends the possibility of preparing new derivatives of new azo-amine by changing aldehyde or amine, and changing some components in order to increase the safe concentration of their use and reduce their toxicity. It also studied the survival of some types of fungi and some types of viruses, as well as other lines of breast cancer cells.