A master's thesis at the College of Science, University of Basrah, discussed the green synthesis of silver nanoparticles (AgNPs), their application in controlling the parasite *Acanthamoeba* spp., and an analysis of their effects based on concentration and exposure time.

The thesis, authored by researcher Zainab Salah Sami, involved the green synthesis of silver nanoparticles using an aqueous extract of rosemary leaves. It also covered the characterization and identification of the nanoparticles to ensure synthesis quality. The study aimed to test the efficacy of the synthesized silver nanoparticles in controlling *Acanthamoeba* spp., a parasite responsible for several serious human diseases. The study concluded that the synthesized silver nanoparticles were highly effective in killing both the trophozoite and cyst stages at low concentrations, showing statistically significant results compared to standard treatments. Recommendations included testing the efficacy of other types of silver nanoparticles on these parasitic stages and evaluating the toxicity of the synthesized nanoparticles on human cell lines.